#FLOWJO COMPENSATION MATRIX HOW TO#

To address this challenge, we developed FlowKit, a Gating-ML 2.0-compliant Python package that can read and write FCS files and FlowJo workspaces. To a large extent, this cuts domain experts off from the rapidly growing library of Single Cell Data Science algorithms available, curtailing the potential contributions of these experts to the validation and interpretation of results. Domain experts in cytometry laboratories and core facilities increasingly recognize the need for automated workflows in the face of increasing data complexity, but by and large, still conduct all analysis using traditional applications, predominantly FlowJo.

#FLOWJO COMPENSATION MATRIX HOW TO#

7Duke Human Vaccine Institute, Durham, NC, United StatesĪn important challenge for primary or secondary analysis of cytometry data is how to facilitate productive collaboration between domain and quantitative experts.6Department of Surgery, Duke University Medical Center, Durham, NC, United States.5Duke Immune Profiling Core, Duke University School of Medicine, Durham, NC, United States.4BD Life Sciences - FlowJo, Ashland, OR, United States.3Center for Human Systems Immunology, Duke University Medical Center, Durham, NC, United States.2Department of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC, United States.1Duke Center for AIDS Research, Duke University, Durham, NC, United States.Weinhold 1,5,6, Guido Ferrari 1,3,6 and Cliburn Chan 1,2,3 Scott White 1,2,3*, John Quinn 4, Jennifer Enzor 5,6, Janet Staats 5,6, Sarah M.

Flowjo compensation matrix